ALPHA.SF ULTRA RAB10 PT73, 10K AlphaLISA人和鼠GTP结合蛋白RAB10蛋白73位磷酸化检测试剂盒 -

This control lysate is intended for use with the AlphaLISA SureFire Ultra Human and Mouse Phospho-RAB10 (Thr73) Detection Kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, A549 cells were cultured to confluence in T175 flasks in 10% FBS containing medium, then treated with 250 μg/mL chloroquine for 5 hours and lysed with 10 mL of 1X SureFire Ultra Lysis Buffer.